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Huang T et al

Biochemistry 45 , 49834990 (2006)

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In brief, the isolated tubules (see the Isolation of primary mouse renal tubules section for the isolation protocol) were washed twice with cold NaCl (0.9%) solution

And I took it out of the cage, and I pinned it on the table so I could grab it behind the head so we could euthanize it using animal protocols, Murphy said